Annotated Bibliography On Human Biology Department

ARTICLE MAKE A TITLE Qasid Chaudhry Human Biology Department, University of Toronto, Toronto, ON M5S 3J6 Microfluidic chips allow for the manipulation, at small quantities of neuronal cells. Further, these chips allow for precise temporal and spatial control. This model is one that can be useful in the framework of neurodegeneration. Deleglise et al (2013) fabricated a 3 chamber microfluidic chip. The chambers held one of: coritical neuron soma and dendrites, cortical axons, or striatal neurons. The chip was designed to replicate an oriented neuronal connection from the coritcal to the striatal cells. Through the use of a chemically induced axotomy, by adding fluid to the central chamber (housing the cortical axon), showed that their chip could be used to simulate a lesion in the neuronal network. An emphasis was placed on the protection of synapses, and event that in axotomy studies, traumatic injury and in many neurodegenerative disorders precedes the loss of neuronal cell soma and axons. They then showed that zVAD-fmk, a caspase inhibitor and resveratrol did not show synaptic protect ion, while NAD+ and Y27632, a Rho Kinase inhibitor showed significant synaptic protection, despite the mechanism not being clear. Pointing at potential therapeutic targets for neurodegeneration. This can also be further looked at from the functionality of the chip - it is a useful tool in the evaluation of drugs in the axotomy model they presented. 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